TABLE 1.
Strains, plasmids, and primers
| Strain, plasmid, or primer | Description | Source |
|---|---|---|
| BW30270 | E. coli K-12 MG1655; rph+fnr+ | CGSCc |
| TCM3 | BW30270(pGQ1) | This study |
| TCM7 | BW30270(ΔgutQ) | This study |
| TCM11 | BW30270(ΔkdsD) | This study |
| TCM15 | TCM11(ΔkdsDΔgutQ) | This study |
| TCM18 | TCM15(pGQ1) | This study |
| pGQ1 | E. coli K-12 gutQ inserted into NdeI/BamHI sites of pT7-7; Ampr | This study |
| F | GGTGCTAGAATTCATATGAGTGAAGCACTACTGAACGa | Invitrogen |
| R | GAATTCGGATCCAAGTTAAATAATCCCGGCCTGATAGAAATCCTGCb | Invitrogen |
| GQF | GATCGATGTGATCATAACCGGAGAGAGCAATGAGTGAAGCGTGTAGGCTGGAGCTGCTTC | Invitrogen |
| GQR | CGGCTGGCGAAACGTCTGGGATTGAAGGATTAAATAATCCATTCCGGGGATCCGTCGACC | Invitrogen |
| KDF | GCGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTGTGTAGGCTGGAGCTGCTTCG | Invitrogen |
| KDR | GCGACGCACCTGCTTTGCTCATTGTTGTTTATCCTTGAATCTTTACACTACGGATATGAATATCCTCCTTAG | Invitrogen |
| GDF | ATGAATCAGGTTGCCGTTGTC | Invitrogen |
| GDR | CACCAGATTCACCTGTAGCG | Invitrogen |
a
NdeI site underlined.
b
BamHI site underlined.
c
CGSC, E. coli Genetic Stock Center (CGSC no. 7925).