TABLE 3.

Specific activity of GutD and API

E. coli straina	d-Glucitolb	GutD activityc	API activityc,d
BW30270	−	<1	14 ± 3
	+	242 ± 14	48 ± 5
TCM7	−	<1	13 ± 3
	+	374 ± 13	15 ± 2
TCM11	−	<1	2 ± 1
	+	581 ± 48	46 ± 5
TCM3	−	<1	2,573 ± 78
	+	323 ± 28	2,457 ± 117

^aStrains were grown in M9 minimal medium with 0.2% glycerol as carbon source.

^bd-Glucitol was added at 10 mM to the cultures where indicated (+) 4 hours before harvesting.

^cSpecific activity reported in nmoles/min/mg.

^dValues include KdsD and/or GutQ.