TABLE 4.
Specific activity of GutD in ΔAPI strains
| E. coli straina | d-Glucitolb | A5P (μM) | GutD activityc | API activityc |
|---|---|---|---|---|
| TCM15 | − | 50 | <1 | NDd |
| + | 50 | 278 ± 33 | ND | |
| − | 5 | <1 | ND | |
| + | 5 | 9.8 ± 1 | ND | |
| TCM18 | − | 5 | <1 | 1,366 ± 180 |
| + | 5 | 356 ± 27 | 976 ± 101 |
a
Strains were grown in MOPS minimal medium with 0.2% glycerol and preinduced with 10 μM G6P-5 μM A5P.
b
d-Glucitol was added at 10 mM to the cultures where indicated (+) 4 hours before harvesting.
c
Specific activity reported in nmoles/min/mg.
d
ND, no activity detected.