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Selection of Eryr transformants (0.3 μg/ml erythromycin) on TBAB medium with or without IPTG (1 mM): in the top part of the figure, transformation of PB168 (wt) competent cells with chromosomal DNA of PB1934 (ΩpncB′::pMUTIN4), colonies are visible only on plates containing IPTG. When the same DNA was used to transform PB1932 (ΔyrxA) competent cells, the same number of colonies grew on TBAB plates with or without IPTG (lower part of the figure).
