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. 2005 Oct 6;33(17):e150. doi: 10.1093/nar/gni143

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© The Author 2005. Published by Oxford University Press. All rights reserved

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P.denitrificans genomic library construction. 1: P.denitrificans total DNA is digested with Sau3AI endonuclease. 2: dGTPs are added to the P.denitrificans genomic library fragments. 3: The genomic fragments are ligated to the 5′-phosphorylated linker using DNA ligase. 4: To make the hetero-tail fragments, the linker-ligated fragments are amplified with hetero-ended primers.