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. 2005 Oct 6;33(17):e150. doi: 10.1093/nar/gni143

Figure 6.

Figure 6

Gel mobility shift assay for PCR products from P.denitrificans genomic library mixture beads. (A) Before sorting of P.denitrificans genomic library beads (PhaR target fragment:P.denitrificans genomic library fragment = 1:30 000). (B) After sorting of P.denitrificans genomic library beads (PhaR target fragment: P.denitrificans genomic library fragment = 1:30 000). M, ΦX174/HincII marker; −, minus-purified PhaR; and +, plus-purified PhaR. Circles indicate the shift fragment by PhaR. An asterisk indicates PhaR target fragment. A double asterisk indicates a shifted fragment including a region upstream of moxZ from P.denitrificans.