Extended Data Fig. 5. Additional hGluD2 recordings.

All recordings were performed with a −80 mV holding potential, 37 °C, and 10 s is shown from each example recording unless noted. Also, unless otherwise specified, recordings are from a DPHPC bilayer. a, Example five minute recording of a bilayer with hGluD2 with no ligand treatment (black, n = 29) or a bilayer without hGluD2 after vehicle (GDN buffer) treatment (orange, n = 42). b, Inset i, example recording from hGluD2 in bilayers in the presence of 10 mM GABA. Inset ii, current histogram of hGluD2 GABA responses fit with three components (n = 33 individual traces, R² = 0.9898). Over each gaussian component is the corresponding conductance ± SD. c, Example recording from bilayers with hGluD2 in the presence of 10 mM glycine (n = 10). d, Example recording from bilayers with hGluD2 in the presence of 10 mM glutamate (n = 6). e, Example recording of 100 µM NASPM co-applied with 10 mM D-serine (pink, n = 28) overlayed with 10 mM D-serine treatment in the absence of NASPM (black). f, Recordings from bilayers made of brain lipid total extract. Inset i, five minute recording with hGluD2 with no ligand treatment (black, n = 28) or a bilayer without hGluD2 after vehicle (GDN buffer) treatment (orange, n = 39). Inset ii, example recording in the presence of 10 mM D-serine (n = 26). Inset iii, current histogram of brain lipid bilayer recordings from hGluD2 in the presence of D-serine, displayed as in panel b (R² = 0.9759). g, Recordings from hGluD2_R530k in DPHPC bilayers – inset i, example recording in the presence of 10 mM D-serine (n = 10); inset ii, example recording in the presence of 50 mM D-serine (n = 15; five repeats shown overlayed). h, whole cell recordings of rat GluD2. Inset i, example recording of cells expressing rat GluD2 exposed to 10 mM D-serine at 25 °C, stepped to 37 °C, and stepped back down to 25 °C (n = 5). Inset ii, example recording of lurcher (A654T) rat GluD2 at 25 °C, stepped to 37 °C, and stepped back down to 25 °C (n = 5).