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. 2025 Sep 16;647(8091):1063–1071. doi: 10.1038/s41586-025-09610-x

Fig. 2. hGluD2 bilayer recordings.

Fig. 2

a, Recording without ligands at 37 °C (n = 29 individual traces). Total duration shown is 10 s. b, Recordings (10 s) in the presence of 10 mM d-serine at 37 °C (n = 38 individual traces) and 22 °C (n = 42 individual traces). C, closed; O, open. c, Current histogram of d-serine responses fit with three components (n = 38 individual traces, R2 = 0.9787) and current histogram with co-application of 100 µM NASPM. Above each component is the corresponding conductance ± s.d. Peak amplitude values generated from 10 s event detections (n = 80 from each group). d, Mean conductance ± s.e.m. 37 °C, 30.67 ± 0.24 pS (n = 4,467 events); 22 °C, 29.76 ± 0.48 pS (n = 1,206 events); 37 °C + 2 mM Ca2+, 36.49 ± 0.41 pS (n = 2,242 events); 37 °C versus 22 °C mean conductance P value = 0.2051, 95% CI of difference −0.3463 to 2.173, d.f. = 1,854; 37 °C versus 37 °C + 2 mM Ca2+ mean conductance P value < 0.0001, 95% CI of difference −6.948 to −4.700, d.f. = 3,786. e, Mean PO ± s.e.m. 37 °C, 8.85 ± 1.02% (n = 80 10 s event detections); 22 °C, 1.03 ± 0.14% (n = 142 10 s event detections); 37 °C + 2 mM Ca2+, 6.50 ± 1.03% (n = 49 10 s event detections). 37 °C versus 22 °C PO P value < 0.0001, 95% CI of difference 0.05374 to 0.1027, d.f. = 82.05; 37 °C versus 37 °C + 2 mM Ca2+ PO P value = 0.2374, 95% CI of difference −0.0107 to 0.0578, d.f. = 118.8. Columns represent the mean; error bars (red) represent s.e.m. ****P < 0.0001; NS, not significant.