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. 2025 Sep 16;647(8091):1063–1071. doi: 10.1038/s41586-025-09610-x

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© The Author(s) 2025

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 5 — a, Location of the R710W mutation site in wild-type hGluD2 (between A and B subunits only). Inset shows a magnified view of the interface. b, Ten-second recordings of hGluD2(R710W) leak currents in the absence of ligand. c, Histogram of GluD2(R710W) leak currents fit with three components (n = 23 individual traces, R² = 0.9552). Conductance ± s.d. corresponding to each Gaussian component is shown above the bars. d, Left, cryo-EM map of hGluD2(R710W)_apo-leak with the unsharpened map overlaid. Right, views or slices that are viewed perpendicular to the demarcations on the left. Demarcation iv is shown in f, left. The area outlined in orange is the location of the front-to-back local dimer interface containing R710W. e, View of front-to-back local LBD dimer interfaces between subunits D and C (left) and subunits A and B (right). f, Changes in pore shape. Arrangement of the M3 helices in hGluD2(R710W)_apo-leak (perpendicular to demarcation iv in d) (left) and dilation of the pore from hGluD2(R710W)_apo-closed to hGluD2(R710W)_apo-leak (right).