Table 3.
Statistics of all pKa entries by wildtype and mutant residues.
| Residue Name | Wildtype | Mutant | ||||||
|---|---|---|---|---|---|---|---|---|
| No. | Avg. pKa | Lowest pKa | Highest pKa | No. | Avg. pKa | Lowest pKa | Highest pKa | |
| Asp | 227 | 3.67 | 0.50 | 9.90 | 32 | 5.61 | 2.10 | 9.70 |
| Glu | 238 | 4.21 | 2.10 | 10.50 | 52 | 5.17 | 2.90 | 9.40 |
| His | 193 | 6.60 | 2.50 | 9.19 | 18 | 6.09 | 4.03 | 8.90 |
| Lys | 100 | 10.56 | 8.40 | 12.12 | 22 | 8.13 | 5.30 | 10.90 |
| Tyr | 38 | 10.54 | 9.14 | 11.70 | 3 | 6.62 | 6.28 | 6.86 |
| Cys | 45 | 6.02 | 2.88 | 11.10 | 20 | 6.66 | 3.75 | 10.90 |
| N-term | 17 | 7.67 | 6.91 | 9.14 | 0 | \ | \ | \ |
| C-term | 19 | 3.16 | 2.40 | 4.03 | 0 | \ | \ | \ |
| All | 877 | 5.61 | 0.50 | 12.12 | 147 | 6.05 | 2.10 | 10.90 |