Table 1.

The primers and conditions used to obtain Cryptosporidium and Giardia

Region	PCR	Primers	Size (bp)	Denaturation/annealing/extension	References
LSU	1°	LSU2040F—5′ CGAATAGCGTTATCTTTGCTATTT 3′	500	94 °C/30 s 58 °C/30 s 72 °C/50 s 35 cycles	[29]
		LSU3020R—5′ GTCTTCCGCGAAGATCAG 3′
	2°	LSU2065F—5′ TTACCATGGAAT(C/T)AGTTCAGC 3′		94 °C/30 s 58 °C/30 s 72 °C/50 s 35 cycles
		LSU2557R—5′ AACACCATTTTCTGGCCATC 3′
SSU	1°	18SiCF2—5′ GACATATCATTCAAGTTTCTGACC 3′	800	94 °C/30 s 58 °C/30 s 72 °C/30 s 45 cycles	[26]
		18SiCR2—5′ CTGAAGGAGTAAGGAACAACC 3′
	2°	18SiCF1—5′ CCTATCAGCTTTAGACGGTAGG 3′		94 °C/30 s 58 °C/30 s 72 °C/30 s 45 cycles
		18SiCR1—5′ TCTAAGAATTTCACCTCTGACTG 3′
SSU	1°	SSU-F2—5′ TTCTAGAGCTAATACATGCG 3′	830	94 °C/45 s 55 °C/45 s 72 °C/60 s 35 cycles	[25, 71, 86]
		SSU-R2—5′ CCCATTTCCTTCGAAACAGGA 3′
	2°	SSU-F3—5′ GGAAGGGTTGTATTTATTAGATAAAG 3′		94 °C/45 s 55 °C/45 s 72 °C/60 s 35 cycles
		SSU-R4—5′ CTCATAAGGTGCTGAAGGAGTA 3′
gp60	1°	gp15-ATG—5′ ATGAGATTGTCGCCTCATTATC 3′	1000	94 °C/30 s 55 °C/45 s 72 °C/60 s 35 cycles	[31]
		gp15-STOP—5′ TTACAACACGAATAAGGCTGC 3′
	2°	gp15-15A—5′ GCCGTTCCACTCAGAGGAAC 3′		94 °C/30 s 55 °C/30 s 72 °C/30 s 35 cycles
		gp15-15E—5′ CCACATTACAAATGAAGTGCCGC 3′
tpi	1°	AL3543—5′ AAATTATGCCTGCTCGTCG 3′	530	94 °C/45 s 50 °C/45 s 72 °C/60 s 35 cycles	[32]
		AL3546—5′ CAAACCTTTTCCGCAAACC 3′
	2°	AL3544—5′ CCCTTCATCGGTGGTAACTT 3′		94 °C/45 s 50 °C/30 s 72 °C/60 s 35 cycles
		AL3545—5′ GTGGCCACCACTCCCGTGCC 3′