Fig. 4. Characterization of anti-Aly/REF monoclonal antibody, 11G5. Western blot analysis on total extract of HeLa cells, and the nuclear (N) or cytoplasmic (C) fractions of Xenopus oocytes. To load equal amounts of proteins in each well, the nuclear fraction from 10 oocytes and the cytoplasmic fraction from two oocytes were separated on 12.5% SDS–PAGE.