Table I. Summary of the sequence analysis of the 10q25.3 BAC contig.

	Sequence motifs	CENP-A binding region	Non-binding region	p value	Genome average	(>64%) AT-rich average
Base composition	AT, %	65.4	58.3	<0.001	58.0a	n/a
Repeats	tandem repeatsb, %	5.5	2.3	<0.05	n/a	n/a
	satellitesc, %	0	0	1	n/a	n/a
	LINEs, %	43.7	14.7	<0.05	18.5a	26.2a
	L1, %	40.6	11.0	<0.05	15.0a	23.2a
	SINEs, %	6.3	12.9	<0.001	14.1a	6.3a
	Alus, %	5.1	10.0	<0.001	11.8a	4.8a
	LTRs, %	9.8	6.1	>0.05	7.8a	7.2a
	DNA elements, %	2.9	2.9	>0.05	n/a	n/a
Protein binding motifs	HMG1d,e	25.6	16.2	<0.001	17.7f	n/a
	topo IIe,g	9.3	6.2	>0.05	n/a	n/a
	CENP-Be,h	0	1	n/a	n/a	n/a
Genes	predicted gene densityg,i	0.30	1.1	n/a	2.6j	1.0j
	CpG island densityg,i	0	0.53	n/a	1.4j	0.37j

To facilitate statistical analysis of differences between the two regions, the 330 kb CENP-A binding region was split into four equal segments of 82.5 kb, which were analysed separately. Similarly, the non-binding regions were split into 32 segments of 100 kb and one of 70 kb. Results are averages for each region unless otherwise stated and p values from t-tests comparing both regions are also given. Where known, values for average genomic sequences and genomic AT-rich sequences are quoted in the final two columns.

^aFrom Smit (1999).

^bTandem repeats were classed as being two or more copies of any repeat of unit length ≥2 bp.

^cSatellite repeats (with % homology to consensus): α-satellite (80%); β-satellite (100%); γ-satellite (100%); 48 bp repeat (100%); classical satellites Ia and Ib (100%); classical satellite III (100%, two or more copies in tandem).

^dPer kb.

^e100% homology to consensus.

^fFrom Barry et al. (1999).

^gPer 100 kb.

^hNumber of matches.

ⁱFigures were calculated for whole binding and non-binding regions only.

^jPredicted gene density for the whole genome and for AT-rich G bands (Craig, 1995).

n/a, not applicable/attempted.