Fig. 5. Agonist and Ca²⁺-dependent exocytosis in cells from wild-type and SERCA2^+/– mice. In (A), intact pancreatic acini from wild-type (closed circles) or SERCA2^+/– mice (open squares) were stimulated with the indicated concentrations of carbachol, and the fraction of amylase released was calculated as a percentage of total amylase content in the acini. In (B), pancreatic acini from wild-type (closed circles) or SERCA2^+/– mice (open squares) were permeabilized with SLO in a medium containing 4 mM EGTA and an ATP regeneration system. Ca²⁺-dependent exocytosis was initiated by a 1:1 dilution of the permeabilized cells into media containing different amounts of CaCl₂ to yield the indicated free Ca²⁺ concentrations. The results show the mean ± SEM of the indicated number of experiments. *p <0.05 or better relative to the respective control.