Table I. Saccharomyces cerevisiae strains used.
Strain | Description | Source or reference |
---|---|---|
(1) UTL-7Aa | MATα leu2-3, 112 ura3-52 trp1 | Erdmann et al. (1989) |
(2) FY1679 (S288C) | MATa/MATα ura3-52/ura3-52 trp1Δ63/TRP1 | Winston et al. (1995) |
leu2Δ–1/LEU2 his3Δ200/HIS3 | ||
(3) FY1679α2 | MATα ura3-52 trp1-Δ63 leu2-Δ1 | Winston et al. (1995) |
YPH499 | MATa ade2-101 his3-Δ200 leu2-Δ1 ura3-52 | Sikorski and Hieter (1989) |
trp1-Δ63 lys2-801 | ||
(4) UTL-7Apex13Δ1 | pex13Δ::URA3 | Erdmann and Blobel (1996) |
(5) UTL-7Apex3Δ1 | pex3Δ::LEU2 | Höhfeld et al. (1991) |
(6) UTL-7Apex19Δ1 | pex19Δ::LEU2 | Götte et al. (1998) |
UTL-7Aant1Δ1 | ant1Δ::LEU2 | this study |
yHPR2213 | ant1Δ::Loxp | this study |
yHPR2223 | pat1Δ::kanMX4 | this study |
yHPR2233 | pat1Δ::kanMX4 ant1Δ::Loxp | this study |
yHPR2531 | ANT1–GFP-prA::TRP1 (pHPR129) | this study |
yHPR2545 | pex3Δ::LEU2 ANT1–GFP-prA::TRP1 (pHPR129) | this study |
yHPR2563 | faa2Δ::LEU2 | this study |
yHPR2573 | pat1Δ::kanMX4 faa2Δ::LEU2 | this study |
yHPR2584 | pex13Δ::URA3 ANT1–GFP-prA::TRP1 (pHPR129) | this study |
yHPR2596 | pex19Δ::LEU2 ANT1–GFP-prA::TRP1 (pHPR129) | this study |
aParental genotypes, e.g. UTL-7Apex13Δ1 was derived from (1) UTL-7A.