Figure 2.

KCTD9 overexpression enhances the efficacy of immunotherapy. (A). Micro-CT images obtained using an orthotopic mouse model. Micro-CT images of tumors in mice in the PD-1 alone, and combination of PD-1 and KCTD9-overexpression groups at the indicated weeks after injection MC38 cells. (B) At the end of the experiment, tumor tissues were completely dissected from mice. All tumor tissues from all three groups (NC control group, PD-1 group, and KCTD9-OE plus PD-1) are shown. (C and D) Tumors were measured every 3 days. Tumor volume was estimated using the formula: volume = (length × width2)/2. *P <0.05, ***P <0.001. At the end of the experiment, following a 3-day interval after the last dosage, tumor tissues were excised from mice.