Table 1.

Comparison between scRNA-seq and ST technology.

Characteristic	scRNA-seq	Spatial transcriptome
Resolution	Single-cell level	spot level (multiple cells)
Spatial information	Missing	Retain
Organizational handling	Dissociate into single cells	Tissue section
Advantage	Fine identification of cell types	Spatial relationship retention
Limitations	Lost spatial background	Limited resolution
Application scenarios	Cell atlas construction and rare cell identification	Spatial niche analysis, cell interaction