Table 3.

Validation results of accuracy, precision, and relative expanded uncertainty for the determination of stevioside, rebaudioside C, rebaudioside F, rebaudioside A, and rebaudioside D in beverages and sauces

Compound	Added standard (mg/kg)	Intra-daya		Inter-dayb		Relative expanded uncertainty (%)
		Accuracy (%)c	Precision (%RSD)	Accuracy (%)	Precision (%RSD)
Beverages
Stevioside	5	101.63 ± 1.96	1.93	101.78 ± 2.74	2.69	3.55
	20	99.26 ± 1.32	1.33	98.52 ± 1.06	1.07	2.23
	50	98.33 ± 2.37	2.41	99.22 ± 2.12	2.14	1.90
Rebaudioside C	5	97.92 ± 2.93	2.99	97.43 ± 2.48	2.54	4.09
	20	94.30 ± 1.47	1.56	93.57 ± 1.10	1.18	2.33
	50	93.43 ± 1.13	1.21	93.05 ± 1.34	1.44	2.12
Rebaudioside F	5	94.38 ± 1.85	1.96	94.26 ± 1.98	2.11	3.78
	20	93.95 ± 1.30	1.38	94.50 ± 1.36	1.43	2.26
	50	102.72 ± 1.84	1.79	103.37 ± 2.05	1.98	2.03
Rebaudioside A	5	95.31 ± 1.00	1.05	94.10 ± 1.53	1.63	2.37
	20	95.38 ± 1.76	1.84	94.57 ± 1.42	1.50	2.16
	50	99.15 ± 1.75	1.76	98.46 ± 2.32	2.35	2.04
Rebaudioside D	5	96.90 ± 1.85	1.91	97.71 ± 2.53	2.59	4.48
	20	98.30 ± 1.48	1.51	96.36 ± 1.90	1.97	2.41
	50	100.96 ± 1.69	1.68	100.18 ± 1.57	1.57	2.33
Sauces
Stevioside	5	96.26 ± 1.22	1.27	99.16 ± 1.61	1.62	3.39
	20	98.36 ± 3.26	3.32	97.89 ± 2.42	2.47	2.79
	50	95.63 ± 1.35	1.41	96.09 ± 1.26	1.32	2.20
Rebaudioside C	5	94.39 ± 2.28	2.42	97.68 ± 2.72	2.78	3.88
	20	95.21 ± 4.28	4.50	95.00 ± 3.33	3.51	2.56
	50	92.98 ± 0.95	1.02	93.07 ± 0.86	0.93	2.05
Rebaudioside F	5	101.89 ± 1.41	1.38	100.12 ± 4.11	4.10	3.39
	20	98.84 ± 4.90	4.95	98.53 ± 3.79	3.85	2.55
	50	103.39 ± 1.45	1.40	103.40 ± 1.26	1.22	2.20
Rebaudioside A	5	94.03 ± 3.57	3.80	95.58 ± 2.84	2.98	3.84
	20	94.30 ± 1.66	1.76	95.23 ± 2.33	2.45	2.36
	50	97.18 ± 1.73	1.78	97.35 ± 1.55	1.59	1.95
Rebaudioside D	5	99.70 ± 1.29	1.30	101.87 ± 2.78	2.73	3.93
	20	97.80 ± 3.42	3.49	100.47 ± 3.58	3.56	2.46
	50	102.99 ± 0.30	0.29	102.52 ± 1.65	1.61	1.90

^aAnalysis was conducted six times/day

^bAnalysis was conducted three times on 3 days

^cAverage ± SD