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. 2025 Jul 31;7(11):101542. doi: 10.1016/j.jhepr.2025.101542

Table 2.

Performance of PSMA, Glypican 3, HSP70, and GS alone or in combination for diagnosing HCC in the test cohort (n = 472, including 320 hepatocellular nodules and 152 non-tumour livers) and in the validation cohort (n = 124, including 87 hepatocellular nodules and 37 non-tumour livers).

Markers Minimum positive markers required Test cohort (n = 472)
Validation cohort (n = 124)
Se Spe PPV NPV Accuracy Se Spe PPV NPV Accuracy
PSMA 1 0.80 0.89 0.86 0.84 0.85 0.95 0.77 0.67 0.97 0.83
Glypican 3 1 0.53 0.98 0.95 0.71 0.77 0.41 0.99 0.94 0.77 0.80
HSP70 1 0.61 0.88 0.81 0.73 0.76 0.76 0.53 0.44 0.81 0.60
GS 1 0.25 0.99 0.96 0.62 0.66 0.29 1 1 0.74 0.77
Glypican 3, HSP70, GS 2 0.47 0.99 0.98 0.69 0.76 0.41 0.99 0.94 0.77 0.80
PSMA, Glypican 3, HSP70, GS 2 0.73 0.99 0.99 0.82 0.88 0.85 0.87 0.76 0.92 0.86

Best performance for each metric is highlighted in bold. GS, glutamine synthetase; HSP70, heat shock protein 70; NPV, negative predictive value; PPV, positive predictive value; PSMA, prostate-specific membrane antigen; Se, sensitivity; Spe, specificity.