1. Intra-assay Repeatability of the Newly Developed Assay.

Virus Type		Average copies (μL)	SD	CV%
SARS-CoV-2 N1	CAL1	66.3	4.78	7.18
	CAL2	5.80	1.12	7.18
	CAL3	0.55	0.25	46.1
SARS-CoV-2 N2	CAL1	69.56	4.71	6.77
	CAL2	6.08	1.18	19.5
	CAL3	0.50	0.21	41.7
IAV	CAL1	40.21	2.71	6.75
	CAL2	3.46	0.80	23.2
	CAL3	0.36	0.16	43.7
IBV	CAL1	72.79	4.05	5.57
	CAL2	5.45	0.69	12.6
	CAL3	0.51	0.20	38.1
RSV	CAL1	69.94	3.10	4.43
	CAL2	5.89	0.52	8.87
	CAL3	0.72	0.22	31.1
HAV	CAL1	63.29	3.01	4.75
	CAL2	6.14	0.83	13.5
	CAL3	0.45	0.18	40.8
HEV	CAL1	66.90	2.96	4.42
	CAL2	4.61	0.45	9.84
	CAL3	0.48	0.15	31.4