Figure 6.
Mutational hotspots and overlap
(A) The number of mutational hotspots (SNV and INDELs combined) per cell.
(B) A rainfall plot of the distribution of mutational hotspots across the genome. The plot was generated using the “karyoploteR” package of R.41 Each data point represents a mutational hotspot observed within a single cell.
(C) The ratio of the number of overlapping mutations among cells of the same passage and same cell strain (i.e., animal) to the number of overlapping mutations among all cells of all strains. We added 1 to the denominator values to avoid potential 0. Each data point represents a cell. The p values were estimated using linear mixed-effects models, two-tailed. Boxplot elements are defined as follows: center line indicates median, box limits indicate upper and lower quartiles, and whiskers indicate 1.5× interquartile range.
(D) UMAP plot of the scRNA-seq data.
(E) Somatic mutations found in both scWGS and scRNA-seq data of the same cell strains. The top four genes that are found almost exclusively associated with one-cell clusters are shown.