Fig. 1. Expression and processing of the wild-type and D187N FAF variant PGs. COS (A) or BHK (B) cells were transfected and radiolabeled with [³⁵S]methionine, and PG was immunoprecipitated from cells or medium at the indicated time as described in Materials and methods. Asterisks indicate low levels (<1%) of cross-reacting protein(s). The positions of the 83 kDa (full-length) and 68 kDa fragment (C68) are indicated by arrowheads. (C) Quantitation of data presented in (A) and (B). The results are typical of three independent experiments.