Fig. 6. Furin endocytic trafficking pathways leading to processing of variant PG to C68. BHK cells were co-transfected with the wild-type (ctl) or the D187N FAF variant (ctl, a–c) and the indicated Rab GTPase mutants. Following 6 h of expression, cells were washed and the intracellular or secreted full-length and C68 forms recovered after a 2 h time period were analyzed by immunoblotting (A) and quantitated (B). The results are typical of two independent experiments.