Fig. 1. GCs potently suppress local and systemic inflammatory responses in GR^dim mice. (A) Hematoxylin–eosin-stained sections of subdermal fat tissue derived from the back skin of wild-type (GR^+/+) and GR^dim mice treated with either vehicle acetone (con), 10 nmol PMA (P) or PMA plus 50 µg dexamethasone (P+D) for 6 h. Scale bar: 100 µm. (B) Vehicle acetone (con), 1 nmol PMA (P) or PMA plus 5 µg dexamethasone (P/D) were applied ectopically to the ears of the mice and the swelling measured after 6 h. (C) IL-6 levels in serum of mice treated as described in (A) were determined by ELISA. (D and E) Wild-type (GR^+/+) or GR^dim mice were injected with 100 µg of LPS per mouse and killed at the time points indicated. TNF-α serum levels were measured by ELISA (D) and corticosterone serum levels by RIA (E).