Fig. 6. Overexpression of Dab2 leads to enhanced adhesion of macrophages to laminin and collagen type IV. (A) BMM from ICSBP+/+ and ICSBP–/– mice, and (B) RAW cells stably transfected with a pcDNA vector containing the complete Dab2-cDNA or the empty pcDNA were used in an adhesion assay. Indicated numbers of enzymatically labeled cells were plated on 96-well plates coated with laminin or collagen type IV. After 15 min incubation, non-adherent cells were removed by washing, and the adherent cells were stained and processed according to the manufacturer’s protocol. The graphs show the results of a representative experiment; three experiments were performed in triplicate. (C) Total cell extracts of RAW cells stably transfected with pcDNA-Dab2, untransfected or transfected with the empty vector were subjected to western blotting with a Dab2 antibody. Extracts of 5 × 105 cells per lane were loaded on the gel.