Figure 3.

Analysis of global 5hmC across human tissues with MSA

(A) Schematic of bisulfite-based 5modC (left) vs. direct 5hmC profiling (right), where APOBEC3A selectively deaminates 5mC but not cytosine-5-methylenesulfonate (CMS) adduct.

(B) Ternary plot showing global levels of unmodified cytosine (bottom), 5-mC (left), and 5-hmC (right) across tissues.

(C) Spearman correlation of cell division rate computed with EpiTOC2 (x axis) with mean global 5hmC levels across tissues (y axis).

(D) Mean 5modC (top), 5mC (middle), and 5hmC (bottom) across consensus ChromHMM states, averaged by tissue type.

(E) Scatterplot of the average 5mC/(5mC + unmodified C) ratio vs. binned 5hmC levels across tissues (x axis).