Fig. 7. dMyb directly activates the cycB promoter. (A) Co-transfection assays. Increasing amounts of the 700 bp double-stranded RNA corresponding to the dMyb cDNA were transfected into Schneider cells together with a luciferase reporter construct containing the cyclin B promoter. The cycB–Luc or cycBmut–Luc reporters contained the wild-type or mutant cycB promoter in which the seven AACNG sequences were mutated. Luciferase assays were carried out and data are shown as the average with standard deviation of three independent experiments. (B) Western blotting of dMyb. Total cell lysates were prepared from the Schneider cells transfected with the dMyb double-stranded RNA or the control non-transfected cells. Lysates were separated by SDS–PAGE and western blotting was performed using the anti-dMyb antibody.