Fig. 5.
Rate of WT TTR (1.8 μM tetramer) homotetramer subunit exchange with subunits from homotetramers of FT (1.8 μM tetramer) can be used to follow the rate of tetramer dissociation, because dissociation is rate-limiting for subunit exchange under physiological conditions. Subunit exchange kinetics decrease substantially at an equimolar concentration of genistein (3.6 μM, ▴), whereas, at twice the TTR concentration, genistein completely eliminates tetramer dissociation (7.2 μM, ♦). Percentage exchange is calculated by dividing the concentration of (TTR)2(FT)2 at each time point by its equilibrium concentration in the absence of inhibitor.