Fig. 5.

Localization of the AS inducible rep promoter. Each plasmid contains repC-lacZ translational fusions and various amounts of rep promoter DNA or rep promoter-lacZ transcriptional fusions and were introduced into strain NTL4(pHC090), a Ti plasmid-less strain with a plasmid expressing virA and virG from their native promoters. Base positions at the endpoints of resections refer to distances from the repA gene translation start site. The resulting strains were cultured overnight in the presence or absence of 100 μM AS in acidified AB minimal medium (pH 5.5) and tested for β-galactosidase-specific activity (33).