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. 2005 Sep 29;102(41):14843–14848. doi: 10.1073/pnas.0503458102

Fig. 8.

Fig. 8.

Increase in copy number of repABC-dependent plasmids by phosphoVirG. Strain NTL4(pHC090)(pKP23) was incubated overnight in the presence or absence of 100 μM AS. Purified plasmid DNA was digested with KpnI, which linearizes the minireplicons (upper band of each lane) and cuts pHC090 at two sites, generating two lower bands of each lane. The two pHC090-derived fragments were used to normalize the band intensities of repABC-dependent plasmids. Plasmids pHC121 and pHC122 are derivatives of pKP23 with the indicated alterations in the vir box motif.