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. 2005 Sep 29;102(41):14659–14664. doi: 10.1073/pnas.0503484102

Fig. 2.

Fig. 2.

Disrupted expression of genes encoding cell cycle regulators in Men1+/- pancreatic islets. (A) Conventional RT-PCR analysis of mRNA isolated from Ficoll-gradient-purified pancreatic islets for the indicated markers. Levels of mRNA specifying p19 and actin (a loading control) are not detectably altered in Men1+/- mice. (B) Quantitative RT-PCR on islets from 40-week-old Men+/+ and Men1+/- mice for the indicated markers. (C) Western blot comparing expression of the indicated proteins in purified islets from 40-week-old Men1+/+ and Men1+/- mice. Actin serves as a loading control. (D and E) Immunohistochemical detection of nuclear p27Kip1 (black dots) reveals reduced expression in pancreatic islets of Men1+/- mice. (F and G) Nuclear expression of the transcription factor Nkx6.1 (red) by insulin+ β cells (green) is maintained in Men1+/- mice. (H and I) Nuclear expression of the transcription factor Islet-1 (red) by insulin+ β cells (green) is maintained in Men1+/- mice. [Original magnification: ×40 (D and E) and ×63 (F–I).]