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. 2005 Sep 29;102(41):14659–14664. doi: 10.1073/pnas.0503484102

Fig. 3.

Fig. 3.

Menin promotes methylation of histone H3 associated with chromatin at the p18 and p27 genes. MIN6 cells were transfected with DNA specifying wild-type menin (wild-type), or mutant missense alleles of Men1 derived from human tumors that encode the amino acid substitutions A242V, L22R, or T344R. (A) Anti-menin ChIP was performed by using PP3 and PP4 specific for regions in the p18 promoter, or PP3 and PP4 specific for regions in the p27 promoter. Menin produced from alleles that retain histone methyltransferase activity (wild-type, L22R) associate with these promoters. (B) ChIP analysis using antibodies specific for trimethylated histone H3 Lys-4. (C and D) ChIP analysis using antibodies specific to menin and trimethylated histone H3 Lys-4 performed on isolated islets from Men1+/+ and Men1+/- mice. (E) Comparison of trimethylated histone H3 Lys-4 levels in Men1+/+ and Men1+/- islets by Western blotting. Actin protein served as a loading control.