Table 1. Alkaloid analysis of DH 98-325-6 plants independently transformed with the CYP82E2/RNAi construct (and vector control).
| Sample | % Nicotine* | % Nornicotine* | % Conversion† |
|---|---|---|---|
| CYP82E2/RNAi | |||
| 1 | 3.149 | 0.100 | 2.8 |
| 2 | 2.569 | 0.193 | 7.0 |
| 3 | 2.175 | 0.064 | 2.9 |
| 4 | 3.517 | 0.125 | 3.4 |
| 5 | 1.085 | 0.868 | 44.4 |
| 6 | 0.025 | 2.260 | 98.9 |
| 7 | 0.027 | 1.867 | 98.6 |
| 8 | 2.268 | 0.128 | 5.3 |
| 9 | 2.197 | 0.133 | 5.7 |
| 10 | 2.434 | 0.112 | 4.4 |
| Vector control | |||
| 3 | 1.811 | 1.1735 | 48.9 |
| 11 | 0.290 | 2.090 | 87.8 |
Leaves were treated with ethephon and cured for 7 days.
*
Percentage of leaf dry weight.
†
[% nornicotine/(% nicotine + % nornicotine)] × 100.