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. 2005 Sep 28;102(41):14919–14924. doi: 10.1073/pnas.0506581102

Table 2. Alkaloid analysis of individual Petite Havana plants transformed with CYP82E2, CYP82E3, and CYP82E4v1 constructs or the pBl121 vector control.

Sample % Nicotine* % Nornicotine* % Conversion
Vector control
2 0.673 0.018 2.6
8 0.605 0.014 2.3
10 0.694 0.017 2.4
CYP82E2
1 0.706 0.005 0.7
2 0.814 0.022 2.6
3 0.630 0.010 1.6
4 0.647 0.010 1.5
CYP82E3
1 0.761 0.011 1.4
2 0.507 0.009 1.7
4 0.653 0.015 2.2
5 0.643 0.013 2.0
6 0.521 0.007 1.3
7 0.716 0.015 2.1
8 0.701 0.027 3.7
CYP82E4v1
1 0.005 0.347 98.6
2 0.006 0.255 97.4
3 0.017 0.300 94.6
4 0.010 0.384 97.5

Green leaves were harvested and analyzed without treatment.

*

Percentage of leaf dry weight.

[% nornicotine/(% nicotine + % nornicotine)] × 100.