Table 2. Alkaloid analysis of individual Petite Havana plants transformed with CYP82E2, CYP82E3, and CYP82E4v1 constructs or the pBl121 vector control.
Sample | % Nicotine* | % Nornicotine* | % Conversion† |
---|---|---|---|
Vector control | |||
2 | 0.673 | 0.018 | 2.6 |
8 | 0.605 | 0.014 | 2.3 |
10 | 0.694 | 0.017 | 2.4 |
CYP82E2 | |||
1 | 0.706 | 0.005 | 0.7 |
2 | 0.814 | 0.022 | 2.6 |
3 | 0.630 | 0.010 | 1.6 |
4 | 0.647 | 0.010 | 1.5 |
CYP82E3 | |||
1 | 0.761 | 0.011 | 1.4 |
2 | 0.507 | 0.009 | 1.7 |
4 | 0.653 | 0.015 | 2.2 |
5 | 0.643 | 0.013 | 2.0 |
6 | 0.521 | 0.007 | 1.3 |
7 | 0.716 | 0.015 | 2.1 |
8 | 0.701 | 0.027 | 3.7 |
CYP82E4v1 | |||
1 | 0.005 | 0.347 | 98.6 |
2 | 0.006 | 0.255 | 97.4 |
3 | 0.017 | 0.300 | 94.6 |
4 | 0.010 | 0.384 | 97.5 |
Green leaves were harvested and analyzed without treatment.
Percentage of leaf dry weight.
[% nornicotine/(% nicotine + % nornicotine)] × 100.