Fig. 3.

Influence of RBC-SNO repletion in vivo or ex vivo on hemodynamics and gas exchange in pigs. (A) Temporal correlation of changes in SNO-Hb level (Top), alveolar-arterial (A-a) pO₂ difference (Middle), and mean PVR (Bottom) in pigs inhaling the SNO-generating gas ENO (100 ppm, 15 min). SNO/Hb, mol of SNO per mol of Hb (tetramer). Baseline mean (±SEM) pO₂ and pCO₂ were 91 (±3) and 38 (±2) mm Hg, respectively. (B) RBC-mediated improvement in A-a pO₂ difference in pigs after i.v. infusion of either SNO-depleted or SNO-replete porcine RBCs (P < 0.05). Baseline mean (±SEM) pO₂ and pCO₂ were 97 (±5) and 38 (±1) mm Hg in the group receiving SNO-depleted RBCs, and 99 (±5) and 38 (±1) mm Hg in those receiving SNO-replete RBCs.