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. 2005 Sep 28;102(41):14723–14728. doi: 10.1073/pnas.0507223102

Fig. 3.

Fig. 3.

Change in the phenotype of the T cell adventitial infiltrate. Adventitia of the allograft was selectively isolated by microdissection 10 days (d10) and 1 month (M1) posttransplantation. After digestion in a collagenase I solution, adventitial cells were suspended and analyzed by flow cytometry. (A) The lymphocyte composition in the adventitial infiltrate was evaluated by using anti-αβTCR (R73), anti-CD4 (OX35), and anti-CD8 (OX8) mAbs. (B) Anti-MHC II (RT1.B) mAb was used to study the level of activation of the adventitial CD4+ and CD8+ lymphocyte subpopulations. (C) Anti-CD134 mAb was used to identify cells able to provide help to B cells among adventitial CD4+ and CD8+ lymphocyte subpopulations. (D) The evolution of the polarization of the adventitial T cell infiltrate was followed by measuring the percentage of IFNγ-producing lymphocytes by using combined surface and intracellular staining with anti-αβTCR (R73), anti-CD4 (OX35), anti-CD8 (OX8), and anti-IFNγ mAbs. *, P < 0.05; **, P < 0.01; ***, P < 0.001; d10 vs. M1; each symbol represents an animal, and the bold line represents the mean value.