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. 2005 Oct 3;102(41):14747–14752. doi: 10.1073/pnas.0507355102

Fig. 1.

Fig. 1.

Pf-RBC-induced NK cell activation within PBMC. Freshly isolated human PBMC were cultured alone (0) or in the presence of the NK cell target K562, Pf-RBC, or uninfected RBC (RBC). NK cell activation was analyzed by flow cytometry after gating on CD3-CD56+ NK cells (A-C) or on CD56+ lymphocytes (D). Statistical analyses were done by using a one-tailed Wilcoxon signed rank test. (A) After 20-24 h of coculture, the percentage of CD25+ cells within NK cells (Left) and the mean fluorescence intensity (MFI) of CD69 staining on NK cells (Right) are indicated for 30 healthy donors. Each dot represents the result obtained from one donor. (B) IFN-γ production by NK cells in PBMC was assessed by flow cytometry after coculture with 3D7-RBC or RBC during 20-24 h (Left) or 4 h in the presence of suboptimal doses of IL-12 (Right). Each dot represents the results from one donor. (C) IFN-γ production by NK cells was determined after 4 h of PBMC exposure to RBC infected with various Pf strains in the presence of IL-12. For each experiment, NK cell activation with the 3D7 strain was used as a control and represents 100% of NK cell IFN-γ production. Depending on the strains, 2-14 experiments were performed. Means ± SEM are represented. (D) Lamp1 and Lamp2 mobilization by NK cells (CD3-) or CD56+ T cells (CD3+) in PBMC was assessed by flow cytometry after 4 h of culture in the presence of monensin. One representative experiment of five is shown.