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. 2005 Oct 4;102(41):14599–14604. doi: 10.1073/pnas.0507408102

Fig. 1.

Fig. 1.

nbl, a temperature-sensitive regeneration mutant. (A) Whole-mount WT and nbl caudal fin regenerates at 0 and 7 dpa. nbl shows a clear block in fin regeneration. Red dashed lines demarcate amputation plane. Red arrows show clotted blood in nbl.(B) Sections of regenerating fins stained with hematoxylin at 3, 6, 16, and 24 hpa (33°C). A fluid- or bloodfilled space separates the wound epidermis from mesenchyme in nbl (black arrow). nbl fins fail to form blastema (red arrow; 24 hpa). (C) Heart sections 7 dpa stained with acid fuchsin-orange G to stain fibrin (orange) and collagen (blue) deposits. nbl ventricle did not initiate cardiac regeneration. Yellow dashed line demarcates the amputation plane. (D) Heart regeneration at 12 dpa. Myocyte enhancer factor 2 staining marks cardiomyocytes (red), and BrdUrd (green) identifies cycling cells. A low percentage of cardiomyocytes incoporate BrdUrd (orange) in WT. No evidence of myocyte or nonmyocyte proliferation was observed in nbl hearts.