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. 2005 Oct 4;102(41):14599–14604. doi: 10.1073/pnas.0507408102

Fig. 2.

Fig. 2.

Failed blastema formation in nbl. (A) Whole-mount in situ hybridization and longitudinal sections of msxb in WT and nbl fin regenerates at 24 hpa. msxb (red arrowhead) marks early blastema cells. Note the absence of msxb expression in nbl, indicating failure of early blastema formation. (B) Sections from WT and nbl regenerates immunostained for BrdUrd (green) and DAPI (blue). Fish were treated with BrdUrd for 6 h before harvesting at 24 hpa. No blastema is observed in nbl regenerates at 33°C. However, mesenchymal cell proliferation in nbl is slightly increased. Epidermal cell proliferation in nbl is comparable to WT at 33°C. Data in graph are the mean ± SEM; n = 7; *, P < 0.01. (C) Sections from WT and nbl regenerates immunostained for active Caspase-3 (red) and DAPI (blue). WT and nbl fish regenerated for 16 h at 25°C followed by a 4-h incubation at 33°C. Note there are Caspase-3-positive cells proximal to the amputation plane in nbl. Normally, this is where mesenchymal tissue disorganization occurs, a process that immediately precedes early blastema formation.