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. 2005 Oct 4;102(41):14599–14604. doi: 10.1073/pnas.0507408102

Fig. 5.

Fig. 5.

hsp60 rescues nbl.(A) nbl embryos raised at 33°C develop pericardial effusion and die at 5 dpf. Embryos from homozygous nbl crosses were injected with 1 nl of 200 ng/μl mRNA (hsp60WT, hsp60V324E, or enhanced GFP) and then transferred to 33°C. Blue arrows mark pericardial effusion; red arrow shows normal cardiac anatomy. Note that hsp60WT mRNA rescues the pericardial effusion phenotype. (B) nbl embryos were scored for the presence of pericardial effusion and ability to swim at 5 dpf. Hsp60WT mRNA rescues the nbl phenotype. (C) WT embryos injected with morpholino against hsp60 show pericardial effusion at 3 dpf at 28.5°C. Blue arrows mark pericardial effusion. These results indicate that hsp60 dysfunction, and not heat shock, caused the nbl phenotype.