Fig. 6.

GroEL V300E causes reduced chaperone function. (A) The E. coli strain KY1156 (26) requires functional GroEL for growth. GroEL V300E is analogous to Hsp60 V324E. KY1156 transfected with GroEL V300E decreased growth at 25°C, suggesting this mutation reduces GroEL function. (B) GroEL V300E shows decreased affinity for nonnative form of LDH. LDH activity was measured after addition of nonpermissive solution (without ATP at 42°C) at time 0 in the presence of mutant (blue) or WT GroEL (red). Negative control was without GroEL (orange).