Table 1. Cultured K-ras-deficient FLC do not show enhanced apoptosis rate.

Genotype	Annexin V+;7-AAD–, % in R1 + R2 population	Annexin V+;7-AAD–, % in R3 + R4 population
K-ras+/+ (n = 2)	0.37 ± 0.11	0.25 ± 0.04
K-ras+/– (n = 6)	0.69 ± 0.01	0.26 ± 0.00
K-ras–/– (n = 3)	0.60 ± 0.01	0.33 ± 0.00

TER119^– cells were purified from individual embryos and cultured in vitro for 1 day. The cells were stained with FITC-conjugated anti-mouse CD71 and PE-conjugated anti-mouse TER119, followed by incubation with APC-conjugated Annexin V and 7-AAD. The Annexin V⁺;7-AAD^– population (apoptotic cells) were presented as a percentage of the total TER119^– (R1 + R2) or TER119⁺ (R3 + R4) cells analyzed.