Fig. 1.

HR protein expression in hair follicles during the hair cycle. (A) Schematic representation of the hair cycle depicting changes in follicle structure. (B) Schematic of an anagen-stage hair follicle; relevant structures are indicated. A and B were adapted from ref. 5. (C–F) Immunofluorescent detection of HR protein (red) and keratin 14 (K14) (green). DAPI staining (blue) indicates nuclei. (C) HR protein expression in mouse back skin at P12 and P17. HR is detected in wild type (+/+) hair follicles at P17; the signal is specific because there is no staining in Hr^-/- (-/-) follicles (Right). DP is denoted by white dots. Boxed areas are magnified below. (D) Magnification of region in white box shows HR staining in nuclei (HR/DAPI) and in K14-positive ORS and K14-negative hair bulb (HR/K14). Arrowheads denote nuclear staining. (E) Magnification of region in red box shows HR staining in K14-positive ORS. (F) HR expression through the hair cycle. Shown is immunofluorescent staining for HR (red) and K14 (green). Hair cycle stages are indicated. Arrowheads indicate HR signal. (Scale bars, 50 μm.)