Figure 1.

Structure of 3-dimensional skin cell cultures. (A) Scanning electron micrograph of the Cellfoam 3-dimensional matrix. Image courtesy of Cytomatrix LLC. (B and C) IF demonstrating the morphologies of (B) fibroblasts stained with vimentin antibody and (C) keratinocytes stained with antibodies to cytokeratins when these cells were grown alone on matrices. (D) When grown together, keratinocytes (orange) and fibroblasts (green) occupied distinct sites on the matrices. (E) DCs, identified by intense staining with HLA-DR antibodies, were observed only if bone marrow progenitor cells were added to the matrices. (F) DCs were often found adherent to the surface of vimentin⁺ fibroblasts. Magnification: ×2 (E and F), ×5 (A), ×10 (B–D).