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. 2005 Oct 13;115(11):3239–3249. doi: 10.1172/JCI24731

Figure 4.

Figure 4

T cells produced in skin cell cultures are mature and functional. (A and B) Proliferation of T cells in response to (A) control medium and (B) phytohemagglutinin (PHA). (C–F) Expression of CD69 activation antigen in response to (C) control medium and (D–F) concanavalin A (Con A) by (C and D) total T cells and (E) CD4+ and (F) CD8+ subsets. (G and H) Production of TNF-α by CD3+ cells in response to (G) control medium and (H) concanavalin A treatment. (I and J) Production of (I) IFN-γ and (J) IL-2 in response to concanavalin A treatment. Treatment with control medium is not shown for IFN-γ and IL-2 samples but was identical to TNF-α control shown in G. The percentage of positive cells is shown. (K) Proliferation of T cells generated in skin cell cultures (T cells) in response to allogeneic PBMCs. Proliferation was assayed by BrdU incorporation on day 6 of the MLR. BrdU incorporation was detected via flow cytometry with gating on CD3+ T cells. PBMCs were derived from allogeneic, unrelated donors [PBMCs (A), donor A; PBMCs (B), donor B]. Error bars represent the SD of 3 measurements.