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Inhibition of binding. BDCA-4+ pDCs were cultured overnight in the presence of HIV-1MN or R848 and a neutralizing anti-gp120 mAb, b12 or 17b, versus an irrelevant human IgG1 (A); a neutralizing anti-CD4 antibody versus an irrelevant murine IgG1 (B); or AMD 3100 (C). The presence of IFN-α (ng/ml) in the supernatants was assessed by ELISA.
