FIGURE 3.

Precision of analysis pipeline on test data sets with and without complex background noise. Standard deviations of x, y, z, and intensity estimates (A) of DHPSF images with 15,000 counts and a background of 100 counts and no complex background at a series of z positions are plotted alongside the CRLB (green line). The results of CBNet are plotted as solid squares, while the result of UBNet are shown as empty squares. (B) Precision of CBNet’s estimates for DHPSF images with 15,000 counts over a range of backgrounds generated by the CB algorithm. The CRLB from (A) is plotted as a green line for comparison.