Table I. Light-saturated PSII electron transport rates (µmol O2/ mg Chl/h) in BBY preparations isolated from wild-type and cia3 mutant C.reinhardtii cells.
| Treatment | Wild type | cia3 |
|---|---|---|
| pH 6.5, control | 240 ± 20 | 250 ± 15 |
| pH 6.5 + 1 mM HCO3– | 245 ± 7 | 321 ± 25 |
| pH 5.5, control | 220 ± 6 | 230 ± 10 |
| pH 5.5 + 1 mM HCO3– | 215 ± 14 | 342 ± 8 |
The light-saturated electron flow through PSII in isolated BBY preparations from wild-type and mutant C.reinhardtii cells was measured as O2 evolution in a buffer containing 20 mM MES-KOH, 400 mM sucrose and 35 mM NaCl, at two different pH values, 6.5 and 5.5, in the presence of 1 mM DCBQ and 1 mM K3Fe(CN)6.