Table III. PSII and PSI characteristics of wild-type and cia3 mutant C.reinhardtii cells.

Parameter	Wild type	cia3
PSII/PSI	1.58 ± 0.2	2.4 ± 0.1
Chl a/b	2.4 ± 0.05	2.9 ± 0.08
Chl/PSII	272 ± 13.6	165 ± 6.6
Manganese content (Mn/PSII)a	4.5 ± 0.2	1.9 ± 0.1

Concentrations of PSII and PSI reaction centres in thylakoids were determined from measurements of photoinduced absorbance changes related to the photoreduction of pheophytin and photo-oxidation of P700, respectively. The pigment composition of wild type and mutant was determined spectroscopically. Results are the means ± SE of three independent experiments.

^aManganese content was measured on the basis of chlorophyll molecules per PSII reaction centre (4.5 Mn atoms per 272 Chl molecules in wild type, 1.9 atoms per 165 Chl molecules in mutant).