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Protein expression levels evaluation by WB of COL2A1, ACAN and HAS-2 after 48 h (A) and 96 h (B) of GAG-based treatments. Protein expression levels evaluation by WB of COMP-2, NF-κB and MMP-13 after 48 h (C) and 96 h (D) of GAG-based treatments. Densitometric analyses were performed by normalizing each protein expression vs. ACTIN. Data are expressed as mean ± SD. Statistical analysis was performed using a t-test: *p < 0.05 vs. pCTR; #p < 0.05 for HHA/BC+PRP vs. HHA/BC.
